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Catalog number: SBB-TR0051-2K, 2000 wells
Mixture of Europium Cryptate Ubiquitin (SBB-TR0014), Cy5-Ubiquitin (SBB-TR0015), and wild-type Ubiquitin (SBB-UP0013) combined in a ratio optimized for TR-FRET based conjugation experiments where long polyubiquitin chains are formed. Conjugation reactions are monitored at Excitation = 304 nm, Emission channel 1 = 620 nm, Emission channel 2 = 665 nm. These proteins were expressed in E.coli.
Catalog number: SBB-TR0015, 50 μg
Highly purified recombinant protein, which has been labeled with a single Cyanine5 (Cy5) moiety at a specific site that keeps all lysines within the protein available and has a fully functional C-terminus that allows for further conjugation into ubiquitin chains and does not affect or impairs the E1 – E2 and E3 conjugation cascade. Cy5-Ubiquitin has an excitation maximum Ex646nm and an emission maximum Em662nm.
Catalog number: SBB-TR0016, 50 μg
Highly purified recombinant protein, which has been labeled with a single fluorescein (6-FAM) moiety at a specific site that keeps all lysines within the protein available and has a functional C-terminus. Allows for conjugation into ubiquitin chains and does not affect or impair the E1 – E2 and E3 conjugation cascade. 6-FAM Ubiquitin has an excitation maximum Ex494nm and an emission maximum Em520nm.