Catalog number: SBB-PS0008, 2 mg
Ac-WLA-AMC is a fluorogenic peptidyl substrate for measuring chymotrypsin-like activity of the proteasome. Hydrolysis of this substrate by the β5 subunit of the 20S proteasome is monitored by observing fluorescence at an Excitation wavelength of 345nm and Emission at 445nm. [1][2][3]
Ac-Trp-Leu-Ala-AMC (WLA-AMC)
Ac-WLA-AMC (Acetyl-Trp-Leu-Ala-AMC) is a 7-amino-4-methylcoumarin labeled fluorogenic peptidyl substrate hydrolyzed by the β5 subunit of the 20S proteasome. Chymotrypsin-like activity can be measured using a working concentration of 20-50μM substrate. This substrate is specific to the constitutive proteasome, and is not hydrolyzed efficiently by the immunoproteasome. Cleavage of this peptide by the proteasome or other enzymes liberates the fluorophore AMC causing a strong fluorescent signal which is detected at an Excitation wavelength of 345nm and Emission wavelength of 445nm. 20S Proteasome enzyme requires activation with 0.035% SDS in the assay buffer.