NEDD8-AMC

NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8) is a ubiquitin like protein that plays an important role in regulating development and the cell cycle.  NEDD8 is conjugated to a target protein via a signaling cascade similar to ubiquitin: a NEDD8 specific E1 activating enzyme (APPBP1/UBA3) adenylates the c-terimnus of NEDD8 which is than subsequently passed to an E2 conjugating enzyme (UBE2M or UBE2F) that facilitates covalent attachment of NEDD8 to a cullin subunit of an SCF E3 ubiquitin ligase. NEDD8-Rh 110 can be used as a substrate for enzymes exhibiting deNEDDylating activity, e.g UCH-L3, UCH-L1, COP9 Signalosome, and NEDP1.  This product consists of a full-length, mature NEDD8 polypeptide (amino acids 1-76) recombinantly expressed in E.coli, conjugated on its c-terminus to a quenched AMC110 dye.  Once hydrolyzed the free AMC provides excellent utility for real time assessment of enzyme activity at excitation (345 nm) and emission (445 nm). Typical working range is 50-500nM.