Catalog number: SBB-CE0019, 100 μg
UBE2D3 is an E2 conjugating enzyme which catalyzes the E3 mediated ubiquitination of a target substrate. It is known to interact in conjunction with E3 ligase MDM2 to ubiquitinate p53. This protein is recombinantly expressed in E.coli.[1][2]
UBE2D3, human recombinant
UBE2D3 is an E2 ubiquitin conjugating enzyme. An E1 activating enzyme is required to attach ubiquitin to UBE2D3 via an active site cysteine. The mechanism of ubiquitin transfer involves the breaking of a E1-Ub thioester linkage, followed by a reformation of a UBE2D3-Ub thioester. UBE2D3 is capable of associating with numerous known E3 ligases including MDM2, which target proteins such as p53 for proteasomal degradation through polyubiquitination. This recombinant UBE2D3 is expressed in E.coli. Working concentrations of this enzyme range from 1 to 5µM.