Catalog number: SBB-CE0011, 50 µg
UBA1 is the canonical Ubiquitin E1 activating enzyme, which catalyzes the first step of the Ubiquitin conjugation cascade. UBA1 charges Ubiquitin to a thioester on its active site cysteine through an adenylate intermediate.[1][2][3][4]
Ubiquitin Activating Enzyme (UBA1), human recombinant
UBA1, the canonical Ubiquitin E1 activating enzyme is a 118kDa protein, which forms a homodimer in its active state. It activates Ubiquitin in an ATP-dependent mechanism where ATP is hydrolyzed to AMP and PPi, a Ubiquitin C-terminal adenylate intermediate is formed, then transferred to the E1’s active site cysteine through a thioester bond. This thioester is then transferable to an E2 conjugating enzyme’s active site cysteine. Working concentrations of this enzyme range from 10 to 100nM. This enzyme is produced recombinantly from insect cells.