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UB Conjugation

Catalog number: SBB-CE0022, 100 μg

Recombinant Human UBE2K (HIP2). E2 enzyme which catalyzes free K48-linked polyubiquitin chains. Known to interact with huntingtin and TRIM6. The protein is N-terminally His-tagged, and recombinantly expressed in E.Coli.

Catalog number: SBB-CE0020, 100 μg

UBE2L3. E2 enzyme with specificity towards E3 ligases containing HECT (Homologous to the E6AP Carboxy-terminus) or RBR (Ring in-between ring) domains. This protein is recombinantly expressed in E.coli.

Catalog number: SBB-CE0019, 100 μg

UBE2D3 is an E2 conjugating enzyme which catalyzes the E3 mediated ubiquitination of a target substrate. It is known to interact in conjunction with E3 ligase MDM2 to ubiquitinate p53. This protein is recombinantly expressed in E.coli.

Catalog number: SBB-CE0027, 100 μg

UBE2D2 is an E2 conjugating enzyme which catalyzes the E3 mediated ubiquitination of a target substrate. It is known to interact with Parkin, and is involved in PINK1 mediated mitophagy. This protein is recombinantly expressed in E.coli.

Catalog number: SBB-CE0021, 100 μg

UBE2D1 is an E2 conjugating enzyme which catalyzes the E3 mediated ubiquitination of a target substrate. It is known to interact with Parkin, and is involved in PINK1 mediated mitophagy. This protein is recombinantly expressed in E.coli.

Catalog number: SBB-CE0058, 50 µg

UBA1 is the canonical Ubiquitin E1 activating enzyme, which catalyzes the first step of the Ubiquitin conjugation cascade. UBA1 charges Ubiquitin to a thioester on its active site cysteine through an adenylate intermediate.

Catalog number: SBB-CE0011, 50 µg

 

UBA1 is the canonical Ubiquitin E1 activating enzyme, which catalyzes the first step of the Ubiquitin conjugation cascade. UBA1 charges Ubiquitin to a thioester on its active site cysteine through an adenylate intermediate.

Catalog number: SBB-UP0013,10 mg

Ubiquitin, human recombinant, is a small (8.5 kDa) regulatory protein that has been found in almost all tissues of eukaryotic organisms. The addition of ubiquitin to a substrate protein is called ubiquitination or ubiquitylation. Ubiquitination can affect proteins in many ways: it can signal for their degradation via the proteasome, alter their cellular location, affect their activity, and promote or prevent protein interactions.

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  • Protein characterization, enzyme kinetics, IC50 & Ki determination

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