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Ubiquitinated-Substrates

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Catalog number: SBB-US0012, 20 μg

This product consists of His6-p53 protein expressed in E.coli and ubiquitinated by MDM2, and subsequently purified from conjugation-reaction proteins/ enriched post-conjugation. Western blot analysis shows multiple states of poly-ubiquitination, and digestion with USP7 shows ubiquitinated p53 can be deconjugated.

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Custom Biochemistry
  • Assay design & development: TR-FRET, ELISA, Fluorescence Polarization

  • Protein & antibody labeling: Maleimide, NHS, Hydrazide coupling & more

  • Expression & purification: E. coli, Yeast, Insect, Mammalian

  • Protein characterization, enzyme kinetics, IC50 & Ki determination

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