Ubiquitin/UBL Derivatives
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Catalog number: SBB-PS0002, 50 μg
This product consists of a full-length, mature ISG15 polypeptide (amino acids 2-157) recombinantly expressed in E.coli, conjugated on its c-terminus to a quenched Rhodamine110 dye. Hydrolysis of the conjugate results in fluorescence observable by excitation at 485nm and emission at 535nm, which substantially reduces the risk of autofluorescence of compounds in screenings (Hassapien et al., 2007). ]
Catalog number: SBB-PS0003, 50 μg
This product consists of a full-length, mature NEDD8 polypeptide recombinantly expressed in E.coli, conjugated on its c-terminus to a quenched Rhodamine110 dye. Once hydrolyzed the free rhodamine provides excellent utility for real time assessment of enzyme activity at excitation (485 nm) and emission (535 nm).
Catalog number: SBB-PS0044, 50 μg
This product consists of a full-length, mature NEDD8 polypeptide recombinantly expressed in E.coli, conjugated on its c-terminus to AMC. Hydrolysis of the conjugate results in fluorescence observable by excitation at 345nm and emission at 445nm. Typical working range is 50-500nM.
Catalog number: SBB-PS0028, 50 μg
This SUMO1 substrate is C-terminally derivatized with a Glycine-Rhodamine-110 (3,6-diamino-9-(2-carboxyphenyl)) fluorophore. The Gly-Rh110 remains quenched until the amide bond between the C-terminal glycine and the Gly-Rh110 compound is hydrolyzed. The efficiency of quenching combined with the powerful signal upon hydrolysis yields a reagent with unparalleled signal-to-background.
Catalog number: SBB-PS0029, 50 μg
This SUMO2 substrate is C-terminally derivatized with a bis-Glycine-Rhodamine-110 fluorophore. The bis-Gly-Rh110 remains quenched until the amide bond between the C-terminal glycine and the rhodamine compound is hydrolyzed. The efficiency of quenching combined with the powerful signal upon hydrolysis yields an unparalleled signal-to-background.
Catalog number: SBB-PS0001, 50 μg
This product consists of a full-length, mature ubiquitin polypeptide (amino acids 1-76) recombinantly expressed in E.coli, conjugated on its c-terminus to a quenched Rhodamine110 dye. Hydrolysis of the conjugate results in fluorescence observable by excitation at 485nm and emission at 535nm. Typical working range is 50-500nM.
Catalog number: SBB-PS0043, 50 μg
This product consists of a full-length, mature ubiquitin polypeptide (amino acids 1-76) recombinantly expressed in E.coli, conjugated on its c-terminus to AMC. Hydrolysis of the conjugate results in fluorescence observable by excitation at 345nm and emission at 445nm. Typical working range is 50-500nM.
Catalog number: SBB-PS0043, 50 μg
This product consists of a full-length, mature ubiquitin polypeptide (amino acids 1-76) recombinantly expressed in E.coli, conjugated on its c-terminus to AMC. Hydrolysis of the conjugate results in fluorescence observable by excitation at 345nm and emission at 445nm. Typical working range is 50-500nM.